ATG7 antibody (AA 71-645)

Catalog No. ABIN7970666

The Rabbit Polyclonal anti-ATG7 antibody (ABIN7970666) specifically detects ATG7 in WB, IHC, ELISA, FACS, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for ATG7 antibody (AA 71-645) (ABIN7970666)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-ATG7 Antibody

Binding Specificity: AA 71-645

Purpose: Anti-ATG7 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human ATG7 shares 92.9% amino acid (aa) sequence identity with both mouse,rat ATG7.

Characteristics: Anti-ATG7 Antibody. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ATG7 recombinant protein (Position: D71-K645). Human ATG7 shares 92.9% amino acid (aa) sequence identity with both mouse and rat ATG7.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: ATG7

Background:

Background: Autophagy related 7 is a protein in humans encoded by ATG7 gene. This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.

Gene Full Name: autophagy related 7

Molecular Weight: 78 kDa

Gene ID: 10533

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy