ACLY antibody (AA 1-180) (Fluoro488)

Catalog No. ABIN7970758

The Mouse Monoclonal anti-ACLY antibody is suitable to detect ACLY in samples from Human, Mouse and Rat. It has been validated for FACS.

Quick Overview for ACLY antibody (AA 1-180) (Fluoro488) (ABIN7970758)

Target: ACLY (ATP Citrate Lyase (ACLY))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACLY antibody is conjugated to Fluoro488

Application: Flow Cytometry (FACS)

Clone: 5I2

Product Details anti-ACLY Antibody (Fluoro488)

Binding Specificity: AA 1-180

Purpose: Anti-ATP citrate lyase ACLY Antibody (monoclonal, 5I2) Fluoro488 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180). Human ATP citrate lyase shares 95% amino acid (aa) sequence identity with both mouse,rat ATP citrate lyase.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180). Human ATP citrate lyase shares 95% amino acid (aa) sequence identity with both mouse and rat ATP citrate lyase.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for ACLY

Target: ACLY (ATP Citrate Lyase (ACLY))

Alternative Name: ACLY

Background:

Background: ATP citrate lyase, aslo known as ACLY, is an enzyme that in animals represents an important step in fatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.

Gene Full Name: ATP citrate lyase

Gene ID: 47

UniProt: P53396

Pathways: Warburg Effect