ACLY antibody (AA 1-180) (HRP)

Catalog No. ABIN7970762

The HRP-conjugated Mouse Monoclonal anti-ACLY antibody (Clone 5I2) (ABIN7970762) specifically detects ACLY in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ACLY antibody (AA 1-180) (HRP) (ABIN7970762)

Target: ACLY (ATP Citrate Lyase (ACLY))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACLY antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 5I2

Product Details anti-ACLY Antibody (HRP)

Binding Specificity: AA 1-180

Purpose: Anti-ATP citrate lyase ACLY Antibody (monoclonal, 5I2) HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180). Human ATP citrate lyase shares 95% amino acid (aa) sequence identity with both mouse,rat ATP citrate lyase.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180). Human ATP citrate lyase shares 95% amino acid (aa) sequence identity with both mouse and rat ATP citrate lyase.

Isotype: IgG2b

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ACLY

Target: ACLY (ATP Citrate Lyase (ACLY))

Alternative Name: ACLY

Background:

Background: ATP citrate lyase, aslo known as ACLY, is an enzyme that in animals represents an important step in fatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.

Gene Full Name: ATP citrate lyase

Gene ID: 47

UniProt: P53396

Pathways: Warburg Effect