AIRE antibody (AA 1-120)

Catalog No. ABIN7971602

The Rabbit Polyclonal anti-AIRE antibody (ABIN7971602) specifically detects AIRE in WB, IF, IHC, ICC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for AIRE antibody (AA 1-120) (ABIN7971602)

Target: AIRE (Autoimmune Regulator (AIRE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIRE antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-AIRE Antibody

Binding Specificity: AA 1-120

Purpose: Anti-AIRE Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human AIRE shares 87.2% amino acid (aa) sequence identity with mouse AIRE.

Characteristics: Anti-AIRE Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human AIRE recombinant protein (Position: M1-K120). Human AIRE shares 87.2% amino acid (aa) sequence identity with mouse AIRE.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Alternative Name: AIRE

Background:

Background: The autoimmune regulator (AIRE) is a protein that in humans is encoded by the AIRE gene. This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CREB binding protein. The encoded protein plays an important role in immunity by regulating the expression of autoantigens and negative selection of autoreactive T-cells in the thymus. Mutations in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy with candidiasis and ectodermal dystrophy (APECED).

Gene Full Name: autoimmune regulator

Molecular Weight: 58-68 kDa

Gene ID: 326

UniProt: O43918

Pathways: Chromatin Binding