BCLAF1 antibody (AA 293-901)

Catalog No. ABIN7972386

This Rabbit Polyclonal antibody specifically detects BCLAF1 in WB, ELISA, IHC, IF, IP, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BCLAF1 antibody (AA 293-901) (ABIN7972386)

Target: BCLAF1 (BCL2-Associated Transcription Factor 1 (BCLAF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCLAF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-BCLAF1 Antibody

Binding Specificity: AA 293-901

Purpose: Anti-BCLAF1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human BCLAF1 shares 94.4% amino acid (aa) sequence identity with mouse BCLAF1.

Characteristics: Anti-BCLAF1 Antibody. Tested in WB, IHC, ICC, IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human BCLAF1 recombinant protein (Position: H293-E901). Human BCLAF1 shares 94.4% amino acid (aa) sequence identity with mouse BCLAF1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BCLAF1

Target: BCLAF1 (BCL2-Associated Transcription Factor 1 (BCLAF1))

Alternative Name: BCLAF1

Background:

Background: This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: BCL2 associated transcription factor 1

Molecular Weight: 150 kDa

Gene ID: 9774

UniProt: Q9NYF8

Pathways: Positive Regulation of Response to DNA Damage Stimulus