BID antibody (C-Term)

Catalog No. ABIN7972739

The Rabbit Polyclonal anti-BID antibody (ABIN7972739) specifically detects BID in WB, IHC and FACS. The antibody is reactive with Mouse and Rat samples.

Quick Overview for BID antibody (C-Term) (ABIN7972739)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BID antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-BID Antibody

Binding Specificity: C-Term

Purpose: Anti-Bid Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat sequence by one amino acid.

Characteristics: Anti-Bid Antibody. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bid, different from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat By Heat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: Bid

Background:

Background: BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al. (1998) mapped the human BID gene to 22q11. Luo et al. (1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.

Gene Full Name: BH3 interacting domain death agonist

Sequence Similarities: Contains 1 C1q domain.

Molecular Weight: 22 kDa

Gene ID: 12122

UniProt: P70444

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity