HLCS antibody (AA 14-654) (Fluoro594)

Catalog No. ABIN7972766

The Fluoro594-conjugated Rabbit Polyclonal anti-HLCS antibody (ABIN7972766) specifically detects HLCS in WB, ELISA, IHC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for HLCS antibody (AA 14-654) (Fluoro594) (ABIN7972766)

Target: HLCS (Biotin-Protein Ligase (HLCS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLCS antibody is conjugated to Fluoro594

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-HLCS Antibody (Fluoro594)

Binding Specificity: AA 14-654

Purpose: Anti-HLCS Antibody Fluoro594 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human HLCS shares 75.9% amino acid (aa) sequence identity with mouse HLCS.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HLCS recombinant protein (Position: Q14-K654). Human HLCS shares 75.9% amino acid (aa) sequence identity with mouse HLCS.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Comment:

Applications are recommended based on the unconjugated antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for HLCS

Target: HLCS (Biotin-Protein Ligase (HLCS))

Alternative Name: HLCS

Background:

Background: This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Gene Full Name: holocarboxylase synthetase

Gene ID: 3141

UniProt: P50747