HLCS antibody (AA 14-654)

Catalog No. ABIN7972772

The Rabbit Polyclonal anti-HLCS antibody (ABIN7972772) specifically detects HLCS in WB, ELISA, FACS and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for HLCS antibody (AA 14-654) (ABIN7972772)

Target: HLCS (Biotin-Protein Ligase (HLCS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLCS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-HLCS Antibody

Binding Specificity: AA 14-654

Purpose: Anti-HLCS Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human HLCS shares 75.9% amino acid (aa) sequence identity with mouse HLCS.

Characteristics: Anti-HLCS Antibody. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HLCS recombinant protein (Position: Q14-K654). Human HLCS shares 75.9% amino acid (aa) sequence identity with mouse HLCS.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HLCS

Target: HLCS (Biotin-Protein Ligase (HLCS))

Alternative Name: HLCS

Background:

Background: This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Gene Full Name: holocarboxylase synthetase

Molecular Weight: 81 kDa

Gene ID: 3141

UniProt: P50747