BUB1 antibody (AA 731-1085) (HRP)

Catalog No. ABIN7973417

The HRP-conjugated Rabbit Polyclonal anti-BUB1 antibody (ABIN7973417) specifically detects BUB1 in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for BUB1 antibody (AA 731-1085) (HRP) (ABIN7973417)

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-BUB1 Antibody (HRP)

Binding Specificity: AA 731-1085

Purpose: Anti-Bub1 AAMP Antibody HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Bub1 shares 81% amino acid (aa) sequence identity with mouse Bub1.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Bub1 recombinant protein (Position: V731-K1085). Human Bub1 shares 81% amino acid (aa) sequence identity with mouse Bub1.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BUB1

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Alternative Name: BUB1

Background:

Background: BUB1, also known as mitotic checkpoint serine/threonine kinase, is an enzyme that in humans is encoded by the BUB1 gene. It is mapped to 2q13. BUB1 is first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression. The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans. Loss-of-function mutations or absence of BUB1 has been reported to result in aneuploidy, chromosomal instability (CIN) and premature senescence. The protein kinase BUB1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase.

Gene Full Name: BUB1 mitotic checkpoint serine/threonine kinase

Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.

Gene ID: 699

UniProt: O43683