BUB1B antibody (AA 26-448)

Catalog No. ABIN7973454

This Rabbit Polyclonal antibody specifically detects BUB1B in WB, IHC, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BUB1B antibody (AA 26-448) (ABIN7973454)

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-BUB1B Antibody

Binding Specificity: AA 26-448

Purpose: Anti-BubR1/BUB1B Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-BubR1/BUB1B Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human BubR1/BUB1B recombinant protein (Position: K26-E448).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, RatImmunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Alternative Name: BUB1B

Background:

Background: Mitotic checkpoint serine/threonine-protein kinase BUB1 beta?is an?enzyme?that in humans is encoded by the?BUB1B?gene. This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

Gene Full Name: BUB1 mitotic checkpoint serine/threonine kinase B

Molecular Weight: 120-130 kDa

Gene ID: 701

UniProt: O60566