REST antibody (AA 401-450)

Catalog No. ABIN747683

The Rabbit Polyclonal anti-REST antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect REST in samples from Human, Rat and Mouse. There are 3+ publications available.

Quick Overview for REST antibody (AA 401-450) (ABIN747683)

Target: REST (RE1-Silencing Transcription Factor (REST))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This REST antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-REST Antibody

Binding Specificity: AA 401-450

Specificity: KO-Validated

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NRSF

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-REST antibody (ABIN747683)

Li, Cheng, Xu, Zhang, Tokat, Dolios, Ramakrishnan, Shen, Wang, Xu: "The transcriptional coactivator Eya1 exerts transcriptional repressive activity by interacting with REST corepressors and REST-binding sequences to maintain nephron progenitor identity." in: Nucleic acids research, Vol. 50, Issue 18, pp. 10343-10359, (2022) (PubMed).

Zhang, Wang, Lu, Wang, Yuan, Yan, Cai, Chang, Zheng, Wu, Zhou: "REST regulates the cell cycle for cardiac development and regeneration." in: Nature communications, Vol. 8, Issue 1, pp. 1979, (2018) (PubMed).

Zhang, Wu, Wang, Wang, Lu, Nechiporuk, Floss, Greally, Zheng, Zhou: "Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse hearts." in: Nucleic acids research, Vol. 45, Issue 6, pp. 3102-3115, (2017) (PubMed).

Target Details for REST

Target: REST (RE1-Silencing Transcription Factor (REST))

Alternative Name: NRSF/REST

Background:

Synonyms: XBR, NRSF, RE1-silencing transcription factor, Neural-restrictive silencer factor, X2 box repressor, REST

Background: Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression.

Gene ID: 5978

UniProt: Q13127

Pathways: Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity