REST antibody (AA 972-1021)

Catalog No. ABIN6742278

The Rabbit Polyclonal anti-REST antibody (ABIN6742278) specifically detects REST in WB. The antibody is reactive with Human and Monkey samples.

Quick Overview for REST antibody (AA 972-1021) (ABIN6742278)

Target: REST (RE1-Silencing Transcription Factor (REST))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This REST antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-REST Antibody

Binding Specificity: AA 972-1021

Specificity: Human REST

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa972-1021 of human REST (Q13127, NP_005603). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Chicken, Platypus (92%), Xenopus (85%), Stickleback (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for REST

Target: REST (RE1-Silencing Transcription Factor (REST))

Alternative Name: NRSF / REST

Background: Name/Gene ID: REST

Synonyms: REST, X2 box repressor, XBR, NRSF

Gene ID: 5978

NCBI Accession: NP_005603

UniProt: Q13127

Pathways: Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity