CTBP1 antibody (C-Term)

Catalog No. ABIN7973696

The Rabbit Polyclonal anti-CTBP1 antibody (ABIN7973696) specifically detects CTBP1 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CTBP1 antibody (C-Term) (ABIN7973696)

Target: CTBP1 (C-terminal Binding Protein 1 (CTBP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-CTBP1 Antibody

Binding Specificity: C-Term

Purpose: Anti-C-terminal-binding protein 1 CtBP1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat,mouse sequences by one amino acid.

Characteristics: Anti-C-terminal-binding protein 1 CtBP1 Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CtBP1, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, MouseWestern blot, 0.1-0.5 μg/mL, Human, Rat, Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CTBP1

Target: CTBP1 (C-terminal Binding Protein 1 (CTBP1))

Alternative Name: CTBP1

Background:

Background: CTBP1, C-terminal-binding protein 1, is a protein that in humans is encoded by the CTBP1 gene. The CtBP1 protein binds to the C-terminus of adenovirus E1A proteins. This gene is mapped to 4p16. This phosphoprotein is a transcriptional repressor (corepressor) and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. CtBP1 and CtBP2 preferentially associate with the E1A via a 5 amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. During skeletal and T cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of delta EF1, a cellular zinc finger-homeodomain protein, and thereby enhances delta EF1-induced transcriptional silencing. In addition, CtBP complexes with CtIP, a 125 kDa protein that recognizes distinctly different protein motifs from CtBP. CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.

Gene Full Name: C-terminal binding protein 1

Molecular Weight: 130 kDa

Gene ID: 1487

UniProt: Q13363

Pathways: Retinoic Acid Receptor Signaling Pathway