IL5RA antibody (AA 11-110)

Catalog No. ABIN747833

The Rabbit Polyclonal anti-IL5RA antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect IL5RA in samples from Human and Mouse. There is 1 publication available.

Quick Overview for IL5RA antibody (AA 11-110) (ABIN747833)

Target: IL5RA (Interleukin 5 Receptor, alpha (IL5RA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL5RA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-IL5RA Antibody

Binding Specificity: AA 11-110

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Cow,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human IL-5R alpha

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-IL5RA antibody (ABIN747833)

Chen, Zhang, Xu, Luan, Piao, Chi, Wang: "Catalpol alleviates ovalbumin-induced asthma in mice: Reduced eosinophil infiltration in the lung." in: International immunopharmacology, Vol. 43, pp. 140-146, (2016) (PubMed).

Target Details for IL5RA

Target: IL5RA (Interleukin 5 Receptor, alpha (IL5RA))

Alternative Name: IL5RA/CD125

Background:

Synonyms: Interleukin-5 receptor alpha, CD125 antigen, CDw125, HSIL5R3, IL5R, IL-5R, Interleukin 5 Receptor alpha, Interleukin 5 receptor subunit alpha, interleukin 5 receptor type 3, MGC26560, IL5RA, IL-5R alpha, IL5RA_HUMAN.

Background: The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3(IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5(IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported.

Gene ID: 3568

UniProt: Q01344

Pathways: JAK-STAT Signaling