Cathepsin L antibody (Middle Region)

Catalog No. ABIN7975964

The Rabbit Polyclonal anti-Cathepsin L antibody (ABIN7975964) specifically detects Cathepsin L in WB, IHC and FACS. The antibody is reactive with Mouse and Rat samples.

Quick Overview for Cathepsin L antibody (Middle Region) (ABIN7975964)

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cathepsin L antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-Cathepsin L Antibody

Binding Specificity: Middle Region

Purpose: Anti-Cathepsin L/MEP/Ctsl Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Cathepsin L/MEP/Ctsl Antibody. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse Cathepsin L/MEP/Ctsl, which shares 70% and 95% amino acid (aa) sequence identity with human CTSL, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Cathepsin L

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Alternative Name: Ctsl

Background:

Background: The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Additionally, this protein cleaves the S1 subunit of the SARS-CoV-2 spike protein, which is necessary for entry of the virus into the cell.

Gene Full Name: cathepsin L

Molecular Weight: 20-42 kDa

Gene ID: 13039

UniProt: P06797

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades