CD58 antibody (AA 29-215)

Catalog No. ABIN7977163

The Rabbit Polyclonal anti-CD58 antibody is suitable to detect CD58 in samples from Human. It has been validated for WB, FACS and IHC.

Quick Overview for CD58 antibody (AA 29-215) (ABIN7977163)

Target: CD58

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD58 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-CD58 Antibody

Binding Specificity: AA 29-215

Purpose: Anti-LFA3/CD58 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-LFA3/CD58 Antibody. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human LFA3 recombinant protein (Position: F29-R215).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Flow Cytometry, 1-3 μg/1x10⁶cells

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CD58

Target: CD58

Alternative Name: CD58

Background:

Background: CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. This adhesion occurs as part of the transitory initial encounters between T cells and Antigen Presenting Cells before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes the T-cell receptors are reactive to. The LFA3 gene is mapped to chromosome 1p13, which is the same location as CD2.

Gene Full Name: CD58 Molecule

Molecular Weight: 26 kDa

Gene ID: 965

UniProt: P19256