CD68 antibody (Middle Region) (HRP)

Catalog No. ABIN7977280

The Rabbit Polyclonal anti-CD68 antibody is suitable to detect CD68 in samples from Mouse and Rat. It has been validated for IHC, WB and ELISA.

Quick Overview for CD68 antibody (Middle Region) (HRP) (ABIN7977280)

Target: CD68 (CD68 Molecule (CD68))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD68 antibody is conjugated to HRP

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Product Details anti-CD68 Antibody (HRP)

Binding Specificity: Middle Region

Purpose: Anti-Macrosialin CD68 Antibody HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat sequence by one amino acid.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse CD68, different from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68

Background:

Background: CD68 is a heavily glycosylated glycoprotein that is highly expressed in macrophages and other mononuclear phagocytes. Macrosialin, if not glycosylated, has the main sequence that consists of 354 amino acids with an expected molecular weight of 37.4 kD. CD68, which can be located in the cytoplasmic granules of a variety of blood cells and myocytes, can be detected via immunohistochemistry. It's particularly effective as a marker for monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts, all of which belong to the macrophage lineage. CD68 is expressed in tissue macrophages and to a lesser extent in dendritic cells. CD68 is involved in tissue macrophage phagocytic activities, as well as intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It works by binding to tissue and organ-specific lectins or selectins, enabling macrophage subsets to be ed to specific locations. Macrophages may be able to crawl over selectin-bearing substrates or other cells if CD68 is rapidly recirculated from endosomes and lysosomes to the plasma membrane.

Gene Full Name: CD68 antigen

Sequence Similarities: Belongs to the LAMP family.

Gene ID: 12514

UniProt: P31996