CDC45 antibody (AA 166-431) (HRP)

Catalog No. ABIN7977941

The HRP-conjugated Mouse Monoclonal anti-CDC45 antibody (Clone 6H6) (ABIN7977941) specifically detects CDC45 in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CDC45 antibody (AA 166-431) (HRP) (ABIN7977941)

Target: CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDC45 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 6H6

Product Details anti-CDC45 Antibody (HRP)

Binding Specificity: AA 166-431

Purpose: Anti-CDC45L Antibody (monoclonal, 6H6) HRP Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human CDC45L recombinant protein (Position: E166-A431).

Isotype: IgG1

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CDC45

Target: CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))

Alternative Name: CDC45

Background:

Background: CDC45 is a protein that in humans is encoded by the CDC45L gene. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.

Gene Full Name: cell division cycle 45

Gene ID: 8318

UniProt: O75419

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA