CENPB antibody (C-Term)

Catalog No. ABIN7978055

The Rabbit Polyclonal anti-CENPB antibody (ABIN7978055) specifically detects CENPB in WB, ICC, IF and FACS. The antibody is reactive with Human samples.

Quick Overview for CENPB antibody (C-Term) (ABIN7978055)

Target: CENPB (Centromere Protein B (CENPB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPB antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-CENPB Antibody

Binding Specificity: C-Term

Purpose: Anti-CENPB Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: identical to the related mouse sequence.

Characteristics: Anti-CENPB Antibody. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CENPB, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CENPB

Target: CENPB (Centromere Protein B (CENPB))

Alternative Name: CENPB

Background:

Background: Centromere protein B also known as major centromere autoantigen B is an autoantigen protein of the cell nucleus. In humans, centromere protein B is encoded by the CENPB gene. This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

Gene Full Name: centromere protein B

Molecular Weight: 80 kDa

Gene ID: 1059

UniProt: P07199