CCT5 antibody (AA 443-535)

Catalog No. ABIN7978297

The Rabbit Polyclonal anti-CCT5 antibody is suitable to detect CCT5 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IP, IF, ICC and FACS.

Quick Overview for CCT5 antibody (AA 443-535) (ABIN7978297)

Target: CCT5 (Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCT5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-CCT5 Antibody

Binding Specificity: AA 443-535

Purpose: Anti-TCP1 epsilon/CCT5 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: coli-derived human CCT5 recombinant protein (Position: L443-K535). Human CCT5 shares 95.7% amino acid (aa) sequence identity with both mouse,rat CCT5.

Characteristics: Anti-TCP1 epsilon/CCT5 Antibody. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human CCT5 recombinant protein (Position: L443-K535). Human CCT5 shares 95.7% amino acid (aa) sequence identity with both mouse and rat CCT5.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CCT5

Target: CCT5 (Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5))

Alternative Name: CCT5

Background:

Background: CCT5 is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP). Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13.

Gene Full Name: chaperonin containing TCP1 subunit 5

Molecular Weight: 60 kDa

Gene ID: 22948

UniProt: P48643