CCR10 antibody (C-Term) (Fluoro488)

Catalog No. ABIN7978829

The Fluoro488-conjugated Rabbit Polyclonal anti-CCR10 antibody (ABIN7978829) specifically detects CCR10 in FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CCR10 antibody (C-Term) (Fluoro488) (ABIN7978829)

Target: CCR10 (Chemokine (C-C Motif) Receptor 10 (CCR10))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCR10 antibody is conjugated to Fluoro488

Application: Flow Cytometry (FACS)

Product Details anti-CCR10 Antibody (Fluoro488)

Binding Specificity: C-Term

Purpose: Anti-GPR2/CCR10 Antibody Fluoro488 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat,mouse sequences by one amino acid.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GPR2/CCR10, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for CCR10

Target: CCR10 (Chemokine (C-C Motif) Receptor 10 (CCR10))

Alternative Name: CCR10

Background:

Background: CCR10, C-C chemokine receptor type 10 is a protein that in humans is encoded by the CCR10 gene. CCR10 is constitutively expressed in skin melanocytes, fibroblasts, and microvascular endothelial cells, and that it is upregulated by IL1B and TNF. Expression was also detected in T cells, Langerhans cells, and peripheral blood mononuclear cells but not in dendritic cells.By fluorescence in situ hybridization, the GPR2 gene is mapped to 17q21.1-q21.3. CCR10 is the receptor for CCL27. CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation. expression of CCL27 in normal keratinocytes and its strong upregulation in skin lesions of atopic dermatitis, contact dermatitis, and psoriasis patients. CCR10+ T lymphocytes were detected in lesional but not normal skin of these patients. Flow cytometric analysis showed that CCL27 binds extracellular matrix components and dermal microvascular endothelial cells and fibroblasts and mediates adhesion and transendothelial migration of CCR10+ circulating leukocytes. CCR10 is predominantly expressed on CD4+CLA+ (cutaneous lymphocyte antigen), rather than CD8+, circulating T cells. RT-PCR, confocal microscopy, and ELISA analysis indicated that keratinocytes exposed to TNF or IL1B but not to IL4 or IFNG in vitro express increased CCL27.

Gene Full Name: C-C motif chemokine receptor 10

Sequence Similarities: Belongs to the G-protein coupled receptor 1 family.

Gene ID: 2826

UniProt: P46092