CARM1 antibody (Middle Region) (HRP)

Catalog No. ABIN7980660

This Rabbit Polyclonal antibody specifically detects CARM1 in WB, ELISA and IHC. It exhibits reactivity toward Human and Rat.

Quick Overview for CARM1 antibody (Middle Region) (HRP) (ABIN7980660)

Target: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARM1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-CARM1 Antibody (HRP)

Binding Specificity: Middle Region

Purpose: Anti-PRMT4/CARM1 Antibody HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related mouse,rat sequences.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PRMT4, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CARM1

Target: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Alternative Name: CARM1

Background:

Background: CARM1 (coactivator-associated arginine methyltransferase 1), also known as PRMT4, is an enzyme encoded by the CARM1 gene found in human beings, as well as many other mammals. This gene is mapped to 19p13.2. CARM1 is a regulator of cyclin E1 and DHFR mRNA expression. Its main function includes catalyzing the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. CARM1 is a secondary coactivator through its association with p160 family (SRC-1, GRIP1, AIB) of coactivators. It is responsible for moving cells toward the inner cell mass in developing blastocysts. This gene also plays an important role in androgen receptors and may play a role in prostate cancer progression.

Gene Full Name: coactivator associated arginine methyltransferase 1

Sequence Similarities: Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family.

Gene ID: 10498

UniProt: Q86X55

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of fat Cell Differentiation