COBRA1 antibody (AA 1-252)

Catalog No. ABIN7980851

The Rabbit Polyclonal anti-COBRA1 antibody (ABIN7980851) specifically detects COBRA1 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for COBRA1 antibody (AA 1-252) (ABIN7980851)

Target: COBRA1 (Cofactor of BRCA1 (COBRA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COBRA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Carrier-free

Product Details anti-COBRA1 Antibody

Binding Specificity: AA 1-252

Purpose: Anti-COBRA1/NELFB Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-COBRA1/NELFB Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human COBRA1 recombinant protein (Position: M1-E252).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for COBRA1

Target: COBRA1 (Cofactor of BRCA1 (COBRA1))

Alternative Name: NELFB

Background:

Background: Cofactor of BRCA1, also known as COBRA1, is a human gene that encodes NELF-B. NELF-B is a subunit of negative elongation factor (NELF), which also includes NELF-A (WHSC2), either NELF-C or NELF-D (TH1L), and NELF-E (RDBP). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1) and SPT5 (SUPT5H), to cause transcriptional pausing of RNA polymerase II. COBRA1 was initially identified in a yeast two-hybrid screen using the BRCT1 domain of BRCA1 as bait.

Gene Full Name: negative elongation factor complex member B

Molecular Weight: 66 kDa

Gene ID: 25920

UniProt: Q8WX92