Cofilin antibody (C-Term) (Fluoro488)

Catalog No. ABIN7980854

The Fluoro488-conjugated Rabbit Polyclonal anti-Cofilin antibody (ABIN7980854) specifically detects Cofilin in FACS. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for Cofilin antibody (C-Term) (Fluoro488) (ABIN7980854)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cofilin antibody is conjugated to Fluoro488

Application: Flow Cytometry (FACS)

Product Details anti-Cofilin Antibody (Fluoro488)

Binding Specificity: C-Term

Purpose: Anti-Cofilin/CFL1 Antibody Fluoro488 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related mouse,rat sequences.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cofilin, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: CFL1

Background:

Background: Cofilin, a member of the ADF/cofilin family, is actually a protein with 70 % sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. It is a ubiquitous actin-binding factor required for the reorganization of actin filaments. The protein is known to sever actin filaments by creating more positive ends on filament fragments, it can cause depolymerization at the minus end of filaments, thereby preventing their reassembly. As a long-lasting in vivo effect, cofilin recycles older ADP-F-actin, helping cell to maintain ATP-G-actin pool for sustained motility. pH , phosphorylation and phosphoinositides regulate cofilin's binding and associating activity with actin.

Gene Full Name: cofilin 1

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Gene ID: 1072

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events