Complement Factor H antibody (AA 136-447)

Catalog No. ABIN7981557

This Rabbit Polyclonal antibody specifically detects Complement Factor H in WB, IHC and FACS. It exhibits reactivity toward Rat and Mouse.

Quick Overview for Complement Factor H antibody (AA 136-447) (ABIN7981557)

Target: Complement Factor H (CFH)

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Complement Factor H antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-Complement Factor H Antibody

Binding Specificity: AA 136-447

Purpose: Anti-Factor H/Cfh Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Factor H/Cfh Antibody. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Factor H/Cfh recombinant protein (Position: N136-T447).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Complement Factor H

Target: Complement Factor H (CFH)

Alternative Name: Cfh

Background:

Background: Factor H is a member of the regulators of complement activation family and is a complement control protein. This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Gene Full Name: complement component factor h

Molecular Weight: 150 kDa

Gene ID: 12628

UniProt: P06909

Pathways: Complement System, Cellular Response to Molecule of Bacterial Origin