Complement Factor I antibody (AA 19-220)

Catalog No. ABIN7981579

The Rabbit Polyclonal anti-Complement Factor I antibody is suitable to detect Complement Factor I in samples from Human and Rat. It has been validated for WB, IHC, ICC and FACS.

Quick Overview for Complement Factor I antibody (AA 19-220) (ABIN7981579)

Target: Complement Factor I (CFI)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Complement Factor I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-Complement Factor I Antibody

Binding Specificity: AA 19-220

Purpose: Anti-Factor I/CFI Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: coli-derived human Factor I recombinant protein (Position: K19-D220). Human Factor I shares 70.7%,71.2% amino acid (aa) sequence identity with mouse,rat Factor I,respectively.

Characteristics: Anti-Factor I/CFI Antibody. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Factor I recombinant protein (Position: K19-D220). Human Factor I shares 70.7% and 71.2% amino acid (aa) sequence identity with mouse and rat Factor I, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, RatImmunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human Immunocytochemistry, 0.5-1 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Complement Factor I

Target: Complement Factor I (CFI)

Alternative Name: CFI

Background:

Background: Complement factor I, also known as C3b/C4b inactivator, is a protein that in humans is encoded by the CFI gene. This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uremic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immune deposits and age-related macular degeneration are other conditions associated with mutations of this gene.

Gene Full Name: complement factor I

Molecular Weight: 75 kDa, 45 kDa

Gene ID: 3426

UniProt: P05156

Pathways: Complement System