CRYAA antibody (AA 1-173)

Catalog No. ABIN7981909

The Mouse Monoclonal anti-CRYAA antibody is suitable to detect CRYAA in samples from Human, Mouse and Rat. It has been validated for WB, IF and ICC.

Quick Overview for CRYAA antibody (AA 1-173) (ABIN7981909)

Target: CRYAA (Crystallin, alpha A (CRYAA))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRYAA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Clone: 10B9

Product Details anti-CRYAA Antibody

Binding Specificity: AA 1-173

Purpose: Anti-Alpha A Crystallin Antibody (monoclonal, 10B9)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: coli-derived human Alpha A Crystallin recombinant protein (Position: M1-S173). Human Alpha A Crystallin shares 94.8% amino acid (aa) sequence identity with both mouse,rat Alpha A Crystallin.

Characteristics: Anti-Alpha A Crystallin Antibody.(monoclonal, 10B9)-2. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Alpha A Crystallin recombinant protein (Position: M1-S173). Human Alpha A Crystallin shares 94.8% amino acid (aa) sequence identity with both mouse and rat Alpha A Crystallin.

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CRYAA

Target: CRYAA (Crystallin, alpha A (CRYAA))

Alternative Name: CRYAA

Background:

Background: Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone, instead they hold them in large soluble aggregates. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed, alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC).

Gene Full Name: crystallin alpha A2

Molecular Weight: 20-23 kDa

Gene ID: 1409

UniProt: P02489

Pathways: M Phase