Cullin 3 antibody (Middle Region) (FITC)

Catalog No. ABIN7982026

The Rabbit Polyclonal anti-Cullin 3 antibody is suitable to detect Cullin 3 in samples from Human, Mouse and Rat. It has been validated for FACS.

Quick Overview for Cullin 3 antibody (Middle Region) (FITC) (ABIN7982026)

Target: Cullin 3 (CUL3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 3 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Product Details anti-Cullin 3 Antibody (FITC)

Binding Specificity: Middle Region

Purpose: Anti-Cullin 3/CUL3 Antibody FITC Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Cullin 3, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Cullin 3

Target: Cullin 3 (CUL3)

Alternative Name: CUL3

Background:

Background: Cullin-3 (Cul 3), is a protein that in humans is encoded by the CUL3 gene. Kipreos et al. (1996) identified a conserved gene family, designated cullins, with at least 5 members in nematodes, 6 in humans, and 3 in S. cerevisiae. Human CUL3 is an ortholog of nematode cul3. Hartz (2012) mapped the CUL3 gene to chromosome 2q36.2 based on an alignment of the CUL3 sequence with the genomic sequence (GRCh37). Sumara et al. (2007) found that KLHL9, KLHL13, and CUL3 interacted ly in a 370-kD protein complex in HeLa cell lysates. Rondou et al. (2008) showed that interaction between KLHL12 and the CUL3 ubiquitin ligase complex ed ubiquitination of dopamine receptor D4 (DRD4). KLHL12 interacted ly with CUL3 and with the polymorphic intracellular loop-3 of D4. By immunoprecipitation analysis of HeLa cells and HCT116 human colon carcinoma cells expressing epitope-tagged proteins, Kigoshi et al. (2011) showed that KLHL7 interacted with CUL3 and ROC1 (RBX1).

Gene Full Name: cullin 3

Sequence Similarities: Belongs to the cullin family.

Gene ID: 8452

UniProt: Q13618

Pathways: M Phase