PPIB antibody (AA 158-216)

Catalog No. ABIN7982560

The Mouse Monoclonal anti-PPIB antibody is suitable to detect PPIB in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ICC, FACS and IF.

Quick Overview for PPIB antibody (AA 158-216) (ABIN7982560)

Target: PPIB (Cyclophilin B (PPIB))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPIB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Carrier-free

Clone: 11C11

Product Details anti-PPIB Antibody

Binding Specificity: AA 158-216

Purpose: Anti-Cyclophilin B PPIB Antibody (monoclonal, 11C11)

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Cyclophilin B PPIB Antibody.(monoclonal, 11C11)-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Cyclophilin B recombinant protein (Position: K158-E216).

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Immunocytochemistry/Immunofluorescence, 2 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PPIB

Target: PPIB (Cyclophilin B (PPIB))

Alternative Name: PPIB

Background:

Background: Peptidyl-prolyl cis-trans isomerase B, also known as CYPB, is an enzyme that in humans is encoded by the PPIB gene. This gene is mapped to 15q22.31. The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta.

Gene Full Name: peptidylprolyl isomerase B

Molecular Weight: 21 kDa

Gene ID: 5479

UniProt: P23284