DBT antibody (AA 46-462) (HRP)

Catalog No. ABIN7985274

This Rabbit Polyclonal antibody specifically detects DBT in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for DBT antibody (AA 46-462) (HRP) (ABIN7985274)

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBT antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-DBT Antibody (HRP)

Binding Specificity: AA 46-462

Purpose: Anti-DBT Antibody HRP Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human DBT shares 89.7% amino acid (aa) sequence identity with mouse DBT.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DBT recombinant protein (Position: K46-R462). Human DBT shares 89.7% amino acid (aa) sequence identity with mouse DBT.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DBT

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Alternative Name: DBT

Background:

Background: The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Gene Full Name: dihydrolipoamide branched chain transacylase E2

Gene ID: 1629

UniProt: P11182