DDOST antibody (AA 1-420)

Catalog No. ABIN7986184

The Rabbit Polyclonal anti-DDOST antibody is suitable to detect DDOST in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA, IP and FACS.

Quick Overview for DDOST antibody (AA 1-420) (ABIN7986184)

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDOST antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-DDOST Antibody

Binding Specificity: AA 1-420

Purpose: Anti-DDOST Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-DDOST Antibody. Tested in WB, IHC, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DDOST recombinant protein (Position: M1-R420).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DDOST

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Alternative Name: DDOST

Background:

Background: This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.

Gene Full Name: dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit

Molecular Weight: 48 kDa

Gene ID: 1650

UniProt: P39656

Pathways: Cell RedoxHomeostasis