NSMCE2 antibody (AA 14-228)

Catalog No. ABIN7986920

The Rabbit Polyclonal anti-NSMCE2 antibody (ABIN7986920) specifically detects NSMCE2 in WB, ELISA, FACS, IF, IP and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NSMCE2 antibody (AA 14-228) (ABIN7986920)

Target: NSMCE2 (E3 SUMO-Protein Ligase NSE2 (NSMCE2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSMCE2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-NSMCE2 Antibody

Binding Specificity: AA 14-228

Purpose: Anti-NSMCE2 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NSMCE2 Antibody. Tested in ELISA, Flow Cytometry, IP, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NSMCE2 recombinant protein (Position: F14-D228).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NSMCE2

Target: NSMCE2 (E3 SUMO-Protein Ligase NSE2 (NSMCE2))

Alternative Name: NSMCE2

Background:

Background: This gene encodes a member of a family of E3 small ubiquitin-related modifier (SUMO) ligases that mediates the attachment of a SUMO protein to proteins involved in nuclear transport, transcription, chromosome segregation and DNA repair. The encoded protein is part of the structural maintenance of chromosomes (SMC) 5/6 complex which plays a key role genome maintenance, facilitating chromosome segregation and suppressing mitotic recombination. A knockout of the orthologous mouse gene is lethal prior to embryonic day 10.5. Naturally occurring mutations in this gene, that abolish the SUMO ligase activity, are associated with primordial dwarfism and extreme insulin resistance.

Gene Full Name: NSE2 SUMO ligase component of SMC5/6 complex

Molecular Weight: 34 kDa

Gene ID: 286053

UniProt: Q96MF7