EVL antibody (AA 268-400)

Catalog No. ABIN7987486

The Rabbit Polyclonal anti-EVL antibody is suitable to detect EVL in samples from Human. It has been validated for WB, ELISA, FACS and IHC.

Quick Overview for EVL antibody (AA 268-400) (ABIN7987486)

Target: EVL (Ena-Vasodilator Stimulated Phosphoprotein (EVL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EVL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-EVL Antibody

Binding Specificity: AA 268-400

Purpose: Anti-EVL Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-EVL Antibody. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human EVL recombinant protein (Position: M268-E400).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EVL

Target: EVL (Ena-Vasodilator Stimulated Phosphoprotein (EVL))

Alternative Name: EVL

Background:

Background: Ena/VASP-like protein is a member of the Ena/VASP family of proteins that in humans is encoded by the EVL gene. Ena/VASP-like (EVL) protein is a member of the Ena/VASP family and is involved in actin-associated cytoskeleton remodeling and cell polarity activities including axon guidance and lamellipodia formation in migrating cells. The EVL protein sequence contains an N-terminal EVH1 domain, a Pro-rich SH3 binding domain, and a C-terminal EVH2 domain. EVL domain interactions with G- and F-actin mediates actin nucleation and polymerization. Research studies have shown that EVL also regulates DNA repair by interaction with RAD51. EVL may function in the DSB repair pathway through the EVH2 domain, which possesses DNA-binding and RAD51 binding activity, thereby coordinating homologous DNA recombination. Research studies have shown EVL expression is up-regulated in human breast cancer associated with clinical stages and may be implicated in invasion and/or metastasis of human breast cancer.

Gene Full Name: Enah/Vasp-like

Molecular Weight: 50 kDa

Gene ID: 51466

UniProt: Q9UI08