ECE1 antibody (AA 18-233)

Catalog No. ABIN7987774

The Rabbit Polyclonal anti-ECE1 antibody is suitable to detect ECE1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and FACS.

Quick Overview for ECE1 antibody (AA 18-233) (ABIN7987774)

Target: ECE1 (Endothelin-Converting Enzyme 1 (ECE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-ECE1 Antibody

Binding Specificity: AA 18-233

Purpose: Anti-ECE1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human ECE1 shares 94%,93.5% amino acid (aa) sequence identity with mouse,rat ECE1,respectively.

Characteristics: Anti-ECE1 Antibody. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ECE1 recombinant protein (Position: M18-T233). Human ECE1 shares 94% and 93.5% amino acid (aa) sequence identity with mouse and rat ECE1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ECE1

Target: ECE1 (Endothelin-Converting Enzyme 1 (ECE1))

Alternative Name: ECE1

Background:

Background: Endothelin converting enzyme 1, also known as ECE1, is an enzyme which in humans is encoded by the ECE1 gene. The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Gene Full Name: endothelin converting enzyme 1

Molecular Weight: 87 kDa

Gene ID: 1889

UniProt: P42892

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling