ENO1 antibody (Middle Region)

Catalog No. ABIN7987818

This Rabbit Polyclonal antibody specifically detects ENO1 in WB, IHC, IP, ICC, IF and FACS. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for ENO1 antibody (Middle Region) (ABIN7987818)

Target: ENO1 (Enolase 1 (ENO1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-ENO1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-ENO1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ENO1 Antibody. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ENO1, which shares 90.9% amino acid (aa) sequence identity with rat ENO1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Monkey, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ENO1

Target: ENO1 (Enolase 1 (ENO1))

Alternative Name: ENO1

Background:

Background: Enolase 1 (ENO1) is a glycolytic enzyme expressed in most tissues. It is mapped to 1p36.23. This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.

Gene Full Name: enolase 1

Molecular Weight: 47 kDa

Gene ID: 2023

UniProt: P06733