ENO1 antibody (Middle Region)

Catalog No. ABIN7987840

The Rabbit Polyclonal anti-ENO1 antibody is suitable to detect ENO1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and FACS.

Quick Overview for ENO1 antibody (Middle Region) (ABIN7987840)

Target: ENO1 (Enolase 1 (ENO1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-ENO1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-ENO1 Antibody

Characteristics: Anti-ENO1 Antibody. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ENO1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ENO1

Target: ENO1 (Enolase 1 (ENO1))

Alternative Name: ENO1

Background:

Background: This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.

Gene Full Name: enolase 1

Molecular Weight: 47 kDa

Gene ID: 2023

UniProt: P06733