DCI antibody (C-Term) (Fluoro647)

Catalog No. ABIN7987916

The Fluoro647-conjugated Rabbit Polyclonal anti-DCI antibody (ABIN7987916) specifically detects DCI in FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for DCI antibody (C-Term) (Fluoro647) (ABIN7987916)

Target: DCI (ECI1) (Enoyl-CoA Delta Isomerase 1 (ECI1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCI antibody is conjugated to Fluoro647

Application: Flow Cytometry (FACS)

Product Details anti-DCI Antibody (Fluoro647)

Binding Specificity: C-Term

Purpose: Anti-DCI/ECI1 Antibody Fluoro647 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse sequence by two amino acids,from the related rat sequence by three amino acids.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DCI, different from the related mouse sequence by two amino acids and from the related rat sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for DCI

Target: DCI (ECI1) (Enoyl-CoA Delta Isomerase 1 (ECI1))

Alternative Name: ECI1

Background:

Background: ECI1/DCI (Dodecenoyl-CoA Delta Isomerase), also known as 3,2-trans-enoyl-CoA isomerase, is an enzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon (position 3) to trans double bonds at beta-carbon (position 2). It plays a particularly important role in the metabolism of unsaturated fatty acids. All classes of enoyl-CoA isomerases belong to a family of enzymes, the hydratase/isomerase or crotonase superfamily, and when examined with x-ray crystallography, exhibit a common structural feature of the family, the N-terminal core with a spiral fold composed of four turns, each turn consisting of two beta-sheets and one alpha-helix. Dodecenoyl-CoA Delta Isomerase is involved in the beta-oxidation, one of the most frequently used pathways in fatty acid degradation, of unsaturated fatty acids with double bonds at odd-numbered carbon positions. It does so by shifting the position of the double bonds in the acyl-CoA intermediates and converting 3-cis or trans-enoyl-CoA to 2-trans-enoyl-CoA.

Gene Full Name: enoyl-CoA delta isomerase 1

Gene ID: 1632

UniProt: P42126