EIF4E antibody (AA 18-217)

Catalog No. ABIN7989055

The Rabbit Polyclonal anti-EIF4E antibody is suitable to detect EIF4E in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IF, FACS and ICC.

Quick Overview for EIF4E antibody (AA 18-217) (ABIN7989055)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4E antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-EIF4E Antibody

Binding Specificity: AA 18-217

Purpose: Anti-EIF4E Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-EIF4E Antibody. Tested in WB, ICC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human EIF4E recombinant protein (Position: E18-V217).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: EIF4E

Background:

Background: The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.

Gene Full Name: eukaryotic translation initiation factor 4E

Molecular Weight: 25 kDa

Gene ID: 1977

UniProt: P06730

Pathways: BCR Signaling