GAR1 antibody (AA 58-165)

Catalog No. ABIN7992805

The Rabbit Polyclonal anti-GAR1 antibody is suitable to detect GAR1 in samples from Human. It has been validated for ELISA, WB, IHC, FACS, IF, ICC and IP.

Quick Overview for GAR1 antibody (AA 58-165) (ABIN7992805)

Target: GAR1 (GAR1 Ribonucleoprotein Homolog (Yeast) (GAR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAR1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Grade: Carrier-free

Product Details anti-GAR1 Antibody

Binding Specificity: AA 58-165

Purpose: Anti-NOLA1/GAR1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NOLA1/GAR1 Antibody. Tested in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NOLA1/GAR1 recombinant protein (Position: F58-K165).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GAR1

Target: GAR1 (GAR1 Ribonucleoprotein Homolog (Yeast) (GAR1))

Alternative Name: GAR1

Background:

Background: H/ACA ribonucleoprotein complex subunit 1 is a protein that in humans is encoded by the GAR1 gene. This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p. Two splice variants have been found for this gene.

Gene Full Name: GAR1 ribonucleoprotein

Molecular Weight: 25 kDa

Gene ID: 54433

UniProt: Q9NY12