GATA2 antibody (AA 23-288)

Catalog No. ABIN7992926

The Rabbit Polyclonal anti-GATA2 antibody (ABIN7992926) specifically detects GATA2 in WB, ELISA, ICC, IF and FACS. The antibody is reactive with Human samples.

Quick Overview for GATA2 antibody (AA 23-288) (ABIN7992926)

Target: GATA2 (GATA Binding Protein 2 (GATA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-GATA2 Antibody

Binding Specificity: AA 23-288

Purpose: Anti-GATA2 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GATA2 Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GATA2 recombinant protein (Position: D23-S288).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GATA2

Target: GATA2 (GATA Binding Protein 2 (GATA2))

Alternative Name: GATA2

Background:

Background: GATA2 (GATA binding protein 2) is a human gene which makes a protein called GATA binding protein 2 - a transcription factor. The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells. Ciciotte et al. (1997)mapped the mouse Gata2 gene to chromosome 6 by study of DNA from an interspecific backcross panel. They pointed out that the human gene had been mapped to chromosome 3 by Dorfman et al. (1992) using DNA from a panel of 12 rodent/human hybrids containing various human chromosomes and applying Southern blot analysis. By exon trapping using a PAC contig spanning a breakpoint region associated with myeloid leukemia, Wieser et al. (2000) mapped the GATA2 gene to chromosome 3q21. GATA2 is transcribed from telomere to centromere. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells.

Gene Full Name: GATA binding protein 2

Molecular Weight: 51 kDa

Gene ID: 2624

UniProt: P23769

Pathways: Stem Cell Maintenance