GFRA1 antibody (AA 25-227)

Catalog No. ABIN7993025

The Rabbit Polyclonal anti-GFRA1 antibody (ABIN7993025) specifically detects GFRA1 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for GFRA1 antibody (AA 25-227) (ABIN7993025)

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFRA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-GFRA1 Antibody

Binding Specificity: AA 25-227

Purpose: Anti-GDNF Receptor alpha 1/GFRA1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human GFRA1 shares 97% amino acid (aa) sequence identity with both mouse,rat GFRA1.

Characteristics: Anti-GDNF Receptor alpha 1/GFRA1 Antibody. Tested in IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GFRA1 recombinant protein (Position: D25-Q227). Human GFRA1 shares 97% amino acid (aa) sequence identity with both mouse and rat GFRA1.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, HumanWestern blot, 0.1-0.5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GFRA1

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Alternative Name: GFRA1

Background:

Background: GDNF family receptor alpha-1 (GFRα1), also known as the GDNF receptor or GFRA1, is a protein that in humans is encoded by the GFRA1 gene. It is mapped to chromosome 10q25.3. The protein encoded by this gene is a member of the GDNF receptor family. GFRA1 is released by neuronal cells, Schwann cells, and injured sciatic nerve. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is also a candidate gene for Hirschsprung disease. Soluble GFRA1 mediates robust recruitment of RET to lipid rafts via a mechanism requiring the RET tyrosine kinase.

Gene Full Name: GDNF family receptor alpha 1

Sequence Similarities: Belongs to the glutamate--cysteine ligase type 3 family.

Molecular Weight: 51 kDa

Gene ID: 14585

UniProt: P56159