GCLC antibody (AA 437-637) (Fluoro550)

Catalog No. ABIN7993922

The Fluoro550-conjugated Rabbit Polyclonal anti-GCLC antibody (ABIN7993922) specifically detects GCLC in FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for GCLC antibody (AA 437-637) (Fluoro550) (ABIN7993922)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLC antibody is conjugated to Fluoro550

Application: Flow Cytometry (FACS)

Product Details anti-GCLC Antibody (Fluoro550)

Binding Specificity: AA 437-637

Purpose: Anti-GCLC Antibody Fluoro550 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human GCLC shares 94% amino acid (aa) sequence identity with both mouse,rat GCLC.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GCLC recombinant protein (Position: E437-N637). Human GCLC shares 94% amino acid (aa) sequence identity with both mouse and rat GCLC.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Alternative Name: GCLC

Background:

Background: GCLC, also named Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Gene Full Name: glutamate-cysteine ligase catalytic subunit

Sequence Similarities: Belongs to the glutamate--cysteine ligase type 3 family.

Gene ID: 2729

UniProt: P48506

Pathways: Cell RedoxHomeostasis