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IRS1 antibody (pSer307) (HRP)

The Rabbit Polyclonal anti-IRS1 antibody has been validated for ELISA, IHC (p) and IHC (fro). It is suitable to detect IRS1 in samples from Human, Mouse, Rat and Goat.
Catalog No. ABIN749882

Quick Overview for IRS1 antibody (pSer307) (HRP) (ABIN749882)

Target

See all IRS1 Antibodies
IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reactivity

  • 307
  • 242
  • 220
  • 23
  • 18
  • 18
  • 17
  • 16
  • 15
  • 15
  • 2
  • 1
  • 1
Human, Mouse, Rat, Goat

Host

  • 306
  • 15
Rabbit

Clonality

  • 300
  • 21
Polyclonal

Conjugate

  • 131
  • 13
  • 12
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 9
  • 9
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 1
This IRS1 antibody is conjugated to HRP

Application

  • 167
  • 129
  • 104
  • 92
  • 84
  • 72
  • 51
  • 48
  • 23
  • 22
  • 21
  • 7
  • 4
  • 2
  • 1
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Binding Specificity

    • 31
    • 28
    • 28
    • 24
    • 24
    • 20
    • 18
    • 17
    • 16
    • 16
    • 16
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer307

    Specificity

    This phosphorylation site is homologous to Ser302 in Mouse and Rat.

    Cross-Reactivity

    Goat, Human, Mouse, Rat

    Predicted Reactivity

    Dog,Cow,Pig,Horse,Rabbit

    Purification

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of Ser307 [TE(p-S)IT]

    Isotype

    IgG
  • Application Notes

    WB 1:300-5000
    IHC-P 1:200-400
    IHC-F 1:100-500

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 μg/μL

    Buffer

    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Handling Advice

    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    IRS1 (Insulin Receptor Substrate 1 (IRS1))

    Alternative Name

    IRS-1

    Background

    Synonyms: HIRS-1, Insulin receptor substrate 1, IRS-1, IRS1

    Background: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).

    Gene ID

    3667

    UniProt

    P35568

    Pathways

    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
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