Nucleostemin antibody (AA 45-549)

Catalog No. ABIN7995811

The Rabbit Polyclonal anti-Nucleostemin antibody is suitable to detect Nucleostemin in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IF, FACS and ICC.

Quick Overview for Nucleostemin antibody (AA 45-549) (ABIN7995811)

Target: Nucleostemin (GNL3) (Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleostemin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-Nucleostemin Antibody

Binding Specificity: AA 45-549

Purpose: Anti-Nucleostemin/GNL3 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Nucleostemin/GNL3 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Nucleostemin/GNL3 recombinant protein (Position: R45-V549).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Nucleostemin

Target: Nucleostemin (GNL3) (Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3))

Alternative Name: GNL3

Background:

Background: Guanine nucleotide-binding protein-like 3, also known as nucleostemin, is a protein that in humans is encoded by the GNL3 gene. The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene.

Gene Full Name: G protein nucleolar 3

Molecular Weight: 65 kDa

Gene ID: 26354

UniProt: Q9BVP2