H2AFY antibody (AA 180-301) (HRP)

Catalog No. ABIN7995939

This Rabbit Polyclonal antibody specifically detects H2AFY in WB, IHC and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for H2AFY antibody (AA 180-301) (HRP) (ABIN7995939)

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFY antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-H2AFY Antibody (HRP)

Binding Specificity: AA 180-301

Purpose: Anti-H2AFY/MACROH2A1 Antibody HRP Conjugated

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human MACROH2A1 shares 97.5% amino acid (aa) sequence identity with both mouse,rat MACROH2A1.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human H2AFY/MACROH2A1 recombinant protein (Position: A180-K301). Human MACROH2A1 shares 97.5% amino acid (aa) sequence identity with both mouse and rat MACROH2A1.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for H2AFY

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Alternative Name: MACROH2A1

Background:

Background: Core histone macro-H2A.1 is a protein that in humans is encoded by the H2AFY gene. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene Full Name: macroH2A.1 histone

Gene ID: 9555

UniProt: O75367