H2AFY antibody (AA 180-301)

Catalog No. ABIN7995943

The Rabbit Polyclonal anti-H2AFY antibody is suitable to detect H2AFY in samples from Human, Rat and Mouse. It has been validated for WB, IHC, IF, ELISA and ICC.

Quick Overview for H2AFY antibody (AA 180-301) (ABIN7995943)

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFY antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-H2AFY Antibody

Binding Specificity: AA 180-301

Purpose: Anti-H2AFY/MACROH2A1 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human MACROH2A1 shares 97.5% amino acid (aa) sequence identity with both mouse,rat MACROH2A1.

Characteristics: Anti-H2AFY/MACROH2A1 Antibody. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human H2AFY/MACROH2A1 recombinant protein (Position: A180-K301). Human MACROH2A1 shares 97.5% amino acid (aa) sequence identity with both mouse and rat MACROH2A1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for H2AFY

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Alternative Name: MACROH2A1

Background:

Background: Core histone macro-H2A.1 is a protein that in humans is encoded by the H2AFY gene. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene Full Name: macroH2A.1 histone

Molecular Weight: 39 kDa

Gene ID: 9555

UniProt: O75367