HSPH1 antibody (AA 653-858)

Catalog No. ABIN7996153

The Mouse Monoclonal anti-HSPH1 antibody is suitable to detect HSPH1 in samples from Human. It has been validated for WB, IHC, ICC, IF and FACS.

Quick Overview for HSPH1 antibody (AA 653-858) (ABIN7996153)

Target: HSPH1 (Heat Shock 105kDa/110kDa Protein 1 (HSPH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Carrier-free

Clone: 3D10

Product Details anti-HSPH1 Antibody

Binding Specificity: AA 653-858

Purpose: Anti-Hsp105/HSPH1 Antibody (monoclonal, 3D10)

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Hsp105/HSPH1 Antibody.(monoclonal, 3D10)-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Hsp105 recombinant protein (Position: Y653-D858).

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HSPH1

Target: HSPH1 (Heat Shock 105kDa/110kDa Protein 1 (HSPH1))

Alternative Name: HSPH1

Background:

Background: HSP105 (HEAT-SHOCK 105/110-KD PROTEIN 1), also called HSPH1 or HSP110, is a protein that in humans is encoded by the HSPH1 gene. Immunohistochemical analysis localizes HSP105 mainly in the cytoplasm. Database analysis indicates that both HSP105 isoforms are highly conserved during evolution. By analysis of radiation hybrids and human/rodent hybrid cell lines, the HSPH1 gene is mapped to chromosome 13. Both HSP105-alpha and HSP105-beta are upregulated in HeLa cells exposed to heat shock. HSP105-alpha, but not HSP105-beta, is also upregulate in response to other cell stresses. Following heat shock, HSP105 relocalizes from a cytoplasmic to perinuclear position. Besides, HSP110 may thus constitute a major determinant for both prognosis and treatment response in colorectal cancer.

Gene Full Name: heat shock protein family H (Hsp110) member 1

Molecular Weight: 105 kDa

Gene ID: 10808

UniProt: Q92598