HBA1 antibody (AA 2-142)

Catalog No. ABIN7996912

The Rabbit Polyclonal anti-HBA1 antibody is suitable to detect HBA1 in samples from Human. It has been validated for WB, IHC and FACS.

Quick Overview for HBA1 antibody (AA 2-142) (ABIN7996912)

Target: HBA1 (Hemoglobin, alpha 1 (HBA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HBA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-HBA1 Antibody

Binding Specificity: AA 2-142

Purpose: Anti-Hemoglobin/HBA1/HBA2 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Hemoglobin shares 85.8% amino acid (aa) sequence identity with mouse Hemoglobin.

Characteristics: Anti-Hemoglobin/HBA1/HBA2 Antibody. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Hemoglobin recombinant protein (Position: V2-R142). Human Hemoglobin shares 85.8% amino acid (aa) sequence identity with mouse Hemoglobin.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HBA1

Target: HBA1 (Hemoglobin, alpha 1 (HBA1))

Alternative Name: HBA1

Background:

Background: The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97 % of the total hemoglobin, alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3 % of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1, some nondeletion alpha thalassemias have also been reported.

Gene Full Name: hemoglobin subunit alpha 1

Molecular Weight: 15 kDa

Gene ID: 3039, 3040

UniProt: P69905