HPSE antibody (AA 206-457) (Biotin)

Catalog No. ABIN7997023

The Rabbit Polyclonal anti-HPSE antibody is suitable to detect HPSE in samples from Mouse. It has been validated for WB, IHC and ELISA.

Quick Overview for HPSE antibody (AA 206-457) (Biotin) (ABIN7997023)

Target: HPSE (Heparanase (HPSE))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPSE antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-HPSE Antibody (Biotin)

Binding Specificity: AA 206-457

Purpose: Anti-Heparanase 1/Hpse Antibody Biotin Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Mouse Heparanase 1 shares 80.2%,96.4% amino acid (aa) sequence identity with human,rat Heparanase 1,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Heparanase 1 recombinant protein (Position: K206-K457). Mouse Heparanase 1 shares 80.2% and 96.4% amino acid (aa) sequence identity with human and rat Heparanase 1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HPSE

Target: HPSE (Heparanase (HPSE))

Alternative Name: Hpse

Background:

Background: Heparanase, also known as HPSE, is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate molecules into shorter chain length oligosaccharides. Heparanase is an endo-beta-D-glucuronidase capable of cleaving heparan sulfate and has been implicated in inflammation and tumor angiogenesis and metastasis. The successful penetration of the endothelial cell layer that lines the interior surface of blood vessels is an important process in the formation of blood borne tumour metastases. Heparan sulfate proteoglycans are major constituents of this layer and it has been shown that increased metastatic potential corresponds with increased heparanase activity for a number of cell lines.

Gene Full Name: heparanase

Gene ID: 15442

UniProt: Q6YGZ1

Pathways: Glycosaminoglycan Metabolic Process