HPSE antibody (AA 206-457)

Catalog No. ABIN7997033

The Rabbit Polyclonal anti-HPSE antibody (ABIN7997033) specifically detects HPSE in WB. The antibody is reactive with Mouse samples.

Quick Overview for HPSE antibody (AA 206-457) (ABIN7997033)

Target: HPSE (Heparanase (HPSE))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPSE antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-HPSE Antibody

Binding Specificity: AA 206-457

Purpose: Anti-Heparanase 1/Hpse Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Mouse Heparanase 1 shares 80.2%,96.4% amino acid (aa) sequence identity with human,rat Heparanase 1,respectively.

Characteristics: Anti-Heparanase 1/Hpse Antibody. Tested in WB applications. This antibody reacts with Mouse.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Heparanase 1 recombinant protein (Position: K206-K457). Mouse Heparanase 1 shares 80.2% and 96.4% amino acid (aa) sequence identity with human and rat Heparanase 1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HPSE

Target: HPSE (Heparanase (HPSE))

Alternative Name: Hpse

Background:

Background: Heparanase, also known as HPSE, is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate molecules into shorter chain length oligosaccharides. Heparanase is an endo-beta-D-glucuronidase capable of cleaving heparan sulfate and has been implicated in inflammation and tumor angiogenesis and metastasis. The successful penetration of the endothelial cell layer that lines the interior surface of blood vessels is an important process in the formation of blood borne tumour metastases. Heparan sulfate proteoglycans are major constituents of this layer and it has been shown that increased metastatic potential corresponds with increased heparanase activity for a number of cell lines.

Gene Full Name: heparanase

Molecular Weight: 65 kDa

Gene ID: 15442

UniProt: Q6YGZ1

Pathways: Glycosaminoglycan Metabolic Process